EXPERIMENTAL STUDY OF TRANSPLANTATION AND THE FATE OF COMPOSITE SKIN COMPRISING MIXED KERATINOCYTES SEEDED ON ACELLULAR DERMAL MATRIX
- VernacularTitle:混合表皮细胞与脱细胞真皮基质构建复合皮的移植和转归研究
- Author:
Zhaofan XIA
;
Shichu XIAO
;
Ju YANG
- Publication Type:Journal Article
- Keywords:
epidermis;
dermis;
coculture;
composite skin
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the fate of composite skin comprising mixed keratinocytes seeded on acellular dermal matrix (ADM) after its transplantation to the wound. Newborn BALB/c and human keratinocytes were mixed in various ratios, seeded on the surface of ADM, and cocultured. The composite skin substitute were then grafted onto the full thickness skin wounds in BALB/c mice. The fate of human keratinocytes was observed. The results showed that the composite skin substitutes could close the full thickness wounds in BALB/c mice. Human keratinocytes were mainly located in the upper layer of the epidermis, and were gradually replaced by BALB/c keratinocytes. This indicated that the mixed culture of keratinocytes of two different species on ADM could close full thickness wounds, having the advantages such as saving the donor skin and shortening the culture time in vitro .
