PRELIMINARY OBSERVATION ON DIFFERENTIATION OF CANINE BONE MARROW STROMAL STEM CELLS INTO MYOGENIC CELLS
- VernacularTitle:狗骨髓基质干细胞体外分化为肌源性细胞的初步观察
- Author:
Jifu CUI
;
Xue ZHAO
;
Zonggu WU
- Publication Type:Journal Article
- Keywords:
bone marrow stromal stem cells;
canine;
culture;
drug induced;
myogenic cell
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
To observe the possibility of differentiation of bone marrow stromal stem cells of adult dog into myogenic cells. In a hope of providing a new cell line for treatment of myocardial infarction and heart failure. Bone marrow stromal stem cells were isolated by their adherence to plastic in vitro . 5 azacytidine was used to induce stem cells to differentiate into cardiomyotes in the experimental group, and same volume of culture media was used in the control group. The cultured cells were exarnined with inverted contrast phase microscope and immunochemistry. 4 weeks after induction, the shape of most of cultured cells changed from fusiform into rod like contiguration in experimental group, immunohistochemistry showed that the induced cells were positive for Desmin, ? smooth muscle actin, and Troponin I. The shape of cultured cells remained fusiform in control group, immunohistochemistry showed that they were mildly positive for Desmin and ? smooth muscle actin and negative for Troponin I. Adult canine bone marrow stromal stem cells were able to differentiate into myogenic cells after induction in vitro .
