PRELIMINARY ESTABLISHMENT AND OPTIMIZATION OF TWO-DIMENSIONAL GEL ELECTROPHORESIS FOR PROTEOMICS OF GASTRIC MUCOSA
- VernacularTitle:胃粘膜组织蛋白质组双向凝胶电泳技术的初步建立与优化
- Author:
Xinhua LI
;
Wandai ZHANG
;
Bota WANG
- Publication Type:Journal Article
- Keywords:
proteome;
two dimensional gel electrophoresis;
immobilized pH gradients;
gastric mucosa
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Two dimensional gel electrophoresis(2 DE) is a key technique for proteomics. To analyze the proteome of gastric mucosa and its changes in different diseases. 2 DE technique was established. A series of important factors, such as sample preparation, electrophoresis parameters, choice of IPG gels, concentration of SDS gels, and protocol for staining were optimized to improve the resolution and reproducibility. By using the proper methods describeds above, choosing appropriate sample amount, using pre cast IPG dry strip and casting 13% equal gel, satisfactory 2 DE maps of gastric mucosa were obtained.
