INSTATANEOUS INJURY OF ARTICULAR CARTILAGE SUBJECTED TO HIGH STRESS MECHANICAL LOADS WITH A LOW RATE OF LOADING
- VernacularTitle:低加载速率的高应力载荷对关节软骨的即时损伤作用
- Author:
Sheng ZHANG
;
Changlin HUANG
;
Yuny HU
- Publication Type:Journal Article
- Keywords:
cartilage, articular;
stress, mechanical;
fluorescent dyes;
microscopy, electron, scanning
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
To explore the mode of matrix and cell injury of articular cartilage subjected to high stress mechanical loads with a low rate of loading. Loads characterized by a constant stress rate of 0.26MPa/s to a peak stress between 2MPa and 11MPa being applied to the adult bovine articular cartilage explants was designated as the test groups. No mechanical loads were applied to the control group. All the explants were maintained in culture for the next few days after loads. The spatial patterns and severity of chondrocyte injury were determined using fluorescein diacetate and propidium iodide. Matrix damages were assessed by inspection of the microstructure of the collagen fiber network under SEM. When the peak stress was 5MPa and higher, the rate of cell death in the explants of test groups was significantly greater compared with controls, and the greater peak stress applied, the higher percentage of dead versus total cells was documented, and a more diffuse distribution of dead cells was observed. When the peak stress reached 9MPa, cell death involving full depth of the cartilage was observed in test groups . Damages to the collagen fiber network were not observed both in test and control groups. The results indicated that high stress mechanical loads with a low rate of loading resulted in more severe chonrocytes damage rather than collapse of collagen fiber network.
