Epitope mapping of hepatitis C virus non-structure 3 protein from a 7 peptide phage library by using immobilized specific monoclonal antibody
- VernacularTitle:丙型肝炎病毒非结构蛋白NS3抗原模拟表位的筛选和鉴定st
- Author:
Yanwei ZHONG
;
Jun CHENG
;
Gang WANG
- Publication Type:Journal Article
- Keywords:
Hepatitis C-like virus;
Bpitopes;
Bacteriophages;
Peptide library
- From:
Chinese Journal of Infectious Diseases
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen HCV NS3 mimotopes by using monoclonal antibody and phage peptide library. Methods Using HCV NS3 monoclonal antibody as selective molecule, a 7 mer phage peptide library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing. Results 13 positive clones were chosen for DNA sequencing. From the experiment and sequen-cing comparsion results, one epitope was confirmed as the mimotope of HCV NS3. Conclusions HCV mimotope is obtained by phage peptide library screening.
