Preparation and analgesic effect of α-cobrotoxin-loaded poly(lactide-co-glycolide) microspheres
10.3969/j.issn.1001-1978.2014.05.019
- VernacularTitle:中华眼镜蛇神经毒素微球的制备及镇痛作用研究
- Author:
Yonglong HAO
;
Meirong CHEN
;
Ming HUANG
- Publication Type:Journal Article
- Keywords:
Chinese cobraneurotoxin;
PLGA,micro-spheres;
analgesic;
rat tail-flick test;
nasal mucosa
- From:
Chinese Pharmacological Bulletin
2014;(5):676-678,679
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the preparation of α-cobro-toxin-loaded poly ( lactide-co-glycolide ) microspheres and their analgesic effect. Method The microspheres were prepared by modified water-in-oil-in-oil ( w/o/o ) emulsion solvent evaporation method. A tail flick assay was used to evaluate the analgesic activity of the micro-spheres after nasal administration. Result The micro-spheres with high entrapment efficiency ( >80%) and average diameter of about 25 μm could be prepared by modified water-in-oil-in-oil ( w/o/o ) emulsion solvent evaporation method. A tail flick assy showed the evi-dence for their analgesic effect. Conclusion When Chinese cobraneurotoxin is loaded into microsphere and then the microsphere is administered through nasal cav-ity, its analgesic effect is enhanced significantly.
