Effect of high phosphate on osteocalcin mRNA expression and calcium deposition in vascular smooth muscle cells
- VernacularTitle:高磷对血管平滑肌细胞骨钙素mRNA表达和钙沉积的影响
- Author:
Xiaoyun WANG
;
Weiping LU
;
Xiufen ZHAO
- Publication Type:Journal Article
- Keywords:
Vascular smooth muscle;
Phosphates;
Calcification;
Osteocalcin
- From:
Chinese Journal of Nephrology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of high phosphate - stimulated calcification in vascular smooth muscle cell. Methods The cell culture of bovine aortic smooth muscle was performed. Osteocalcin (OC) mRNA expression and calcium deposition in different phosphate concentrations [normal phosphate (Pi 1. 5mmol/L), high phosphate (2.0mmol/L)] were determined by radioimmunoassay, RT-PCR, and 0-cresolphthalein complexone method respectively. Results After 72 hours,high phosphate treatment of smooth muscle cells(SMC) enhanced the expression of osteocalcin protein [Pi 2. 0 mmol/L versus Pi 1. 5 mmol/L: (15. 03?2. 60) pg/u-g protein versus (2. 98 ?0.84) pg/ug protein, P
