RECOMBINATION AND EXPRESSION OF ENDOTOXIN BINDING PEPTIDE IN E.COLI
- VernacularTitle:内毒素结合肽表达质粒的构建及其在大肠杆菌中的表达
- Author:
Li MA
;
Yousheng LIU
;
Xiaodon WANG
- Publication Type:Journal Article
- Keywords:
endotoxins;
plasmids;
DNA, recombinant;
prokaryotic expression
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Ninty three bp endotoxin binding peptide gene was cloned with PCR from the amino terminal of human LBP cDNA, which was introduced into the expression plasmid Pinpoint Xa 3. After the constructs being transformed into E.coli DH5?, the postive recombinants were identified by restriction enzyme analysis and DNA sequencing. Induced by IPTG, the desired 16.5kD EBP fusion protein was expressed and confirmed with SDS PAGE and Western blotting. These results paved a reasonable way for the study of a new endotoxin binding peptide.
