INHIBITION OF THE AROMATASE ACTIVITY BY INSULIN SENSITIZER, TROGLITAZONE, IN HUMAN OVARIAN GRANULOSA CELLS
- VernacularTitle:胰岛素增敏剂曲格列酮对人卵巢颗粒细胞芳香化酶活性的抑制作用
- Author:
Ming LI
;
Fangling MA
;
Yimin MU
- Publication Type:Journal Article
- Keywords:
peroxisome proliferator activated receptor gamma;
granulosa cells;
aromatase
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
In this study, we aimed to investigate the regulatory effect of troglitazone (TGZ) on aromatase activity (AA) in human ovarian granulosa cells. The granulosa cells obtained from women who underwent in vito fertilization were treated with various concentrations of TGZ and/or retinoid X receptor ligand (RXR) LG100268 (LG), for 24h, and then cellular AA and the level of P450arom mRNA were determined. The treatment of the cells with TGZ for 24 h resulted in a dramatic inhibition of AA in a dose dependent manner. While the treatment with LG alone inhibited AA, the combined treatment with both TGZ and LG caused a much more reduction in AA. The changes in AA by TGZ and/or LG were associated with comparable changes in P450arom mRNA assessed by RT PCR. These results suggested that TGZ directly inhibited AA in human granulosa cells probably via nuclear receptor system which was composed of peroxisome proliferator activated receptor ?(PPAR?):RXR heterodimer.
