DEVELOPMENT OF AVIDIN-BIOTIN QUARTZ CRYSTAL DNA BIOSENSOR
- VernacularTitle:生物素免疫膜石英晶体DNA传感器的研制
- Author:
Zhongming LIU
;
Fang LIU
;
Jiazho LI
- Publication Type:Journal Article
- Keywords:
quartz crystal;
DNA biosensor;
DNA detection
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the possibility of analysis of single strand DNA by using DNA biosensor, we developed a quartz crystal DNA biosensor and studied its performances. The single strand DNA probe was immobilized on the surface of gold filled quartz crystal by biotin immunoreaction. The DNA biosensors were used to detect the complete complementary and single base mismatch complementary single strand DNA in solution. The DNA biosensors were regenerated with 0 1mol/L HCl after hybridization. The hybridization sensitivity of quartz crystal DNA biosensor is 10ng/ml. In the range of 5～40ng/ml, there was a linear correlation between responding signals and concentrations of complementary single strand DNA with a high specificity. The DNA biosensor could be repeatedly used over 5 times by using 0 1mol/L HCl for regeneration.
