3-D (THREE DIMENSIONAL) RECONSTRUCTION OF MICROSTRUCTURE AND ULTRASTRUCTURE OF THE SCIATIC NERVE OF RAT AFTER INJURY
- VernacularTitle:大鼠坐骨神经损伤后显微及超微结构的三维重建
- Author:
Jing CHEN
;
Bingcang LI
;
Hon HUANG
- Publication Type:Journal Article
- Keywords:
peripheral nerves;
regeneration;
3 D reconstruction
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
To reconstruct 3 D images of microstructure and ultrastructure of regenerated sciatic nerve of rats with serial cross sections. The 6 mm defects of the sciatic nerve of 90 Wistar rats were bridge connected with silicone tube and were sampled at 3, 7, 15, 30, 60, 90 days respectively after injury. The images of optical and electronic microscope were taken by micro photograph system and scanner, then they were input into the computer, by which the image registration and segmentation were completed. The direct volume rendering model was used to realize the 3 D reconstruction and display. The results showed the morphological changes in degenerated and regenerated sciatic nerve fibres and their appendicular structures could be observed with the 3 D images. It is suggested that the reconstructing results could reveal the differences between the regenerated and normal sciatic nerve fibres of rats intuitively and visually, so it could be used as a new method in the study of peripheral nerve injury.
