Ddifferent expression of multidrug-resistance and apoptosis related genes in human lung adenocarcinoma parental cell line A_(549) and cisplatin-resistant cell line A_(549) DDP
- VernacularTitle:人肺腺癌亲代与耐顺铂细胞系耐药与凋亡相关基因表达差异及其相关性
- Author:
Xiping LI
;
Xuyi LIU
;
Jie WANG
- Publication Type:Journal Article
- Keywords:
Lung cancer;
Multidrug resistance;
Apoptosis
- From:
Chinese Journal of Respiratory and Critical Care Medicine
2003;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression difference of multidrug resistance and apoptosis related genes and its contribution to mechanisms of multidrug resistance in human lung adenocarcinoma parental cell line A 549 and cisplatin resistant cell line A 549 DDP Methods RT PCR and Immunohistochemical staining were performed to detect the mRNA expressions and protein production of multidrug resistance related protein (MRP),lung resistance protein (LRP),bcl 2,and C erbB 2 in A 549 and A 549 DDP .Results MRP,bcl 2,and C erbB 2 mRNA were expressed in A 549 ,but no LRP mRNA was detected in A 549 MRP,C erbB 2,and LRP mRNA expression levels in A 549 DDP were significant higher than those in A 549 ,otherwise,LRP mRNA was detected in A 549 DDP There was no difference of bcl 2 mRNA expressions between A 549 and A 549 DDP ( P =0 731) Bcl 2 protein expressions were strongly positive both in A 549 and in A 549 DDP MRP protein expressions in A 549 were faint positive,while C erbB 2 and LRP were negative MRP protein expressions in A 549 DDP was median positive C erbB 2 and LRP protein expressions in A 549 DDP were strongly positive Conclusions The heterogenous expression of MRP,C erbB 2,and LRP in A 549 and A 549 DDP may be associated with primary and secondary drug resistance in lung cancer
