LOW RESPONSIVENESS OF CULTURED HUMAN INTESTINAL EPITHELIAL CELLS TO LPS STIMULATION AND ITS POSSIBLE MECHANISM
- VernacularTitle:人肠上皮细胞内毒素低反应性及其机制探讨
- Author:
Daojie ZHANG
;
Jianxin JIANG
;
Yonghua CHEN
- Publication Type:Journal Article
- Keywords:
intestines epithelial cells;
lipopolysaccharide signal transduction associated receptor;
reverse transcriptase-polymerase chain reaction;
RNase protection assay
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the mechanism of low responsiveness of cultured human intestinal epithelial cells (IECs) to LPS stimulation, IL-8 secretion level was detected by ELISA in supernatants harvested after the IECs were treated with LPS for 18h, and TLR4, TLR2, CD14 and MD2 mRNA were detected by reverse transcriptase-polymerase chain reaction(RT-PCR) and RNase protection assay(RPA). It was found that IL-8 was not detectable after IECs were treated with LPS and the expression levels of TLR4, TLR2, CD14 and MD2 mRNA of IECs were low and after exposed to LPS, the expressions of TLR4, CD14 and MD2 mRNA of IECs became further lower, whereas the expression of TLR2 mRNA didn't change obviously. The result suggests that low expressions of LPS signal transduction-associated receptors on IECs may be responsible for low response of IECs to LPS, so it confirms further that TLR4, CD14 and MD2 play important roles in transmembrane LPS signal transduction.
