INHIBITION OF CHLOROQUINE ON IL-6 RELEASE INDUCED SYNERGISTICALLY BY EC DNA AND ENDOTOXIN
- VernacularTitle:氯喹抑制EC DNA、内毒素协同诱导的IL-6释放
- Author:
Hong ZHOU
;
Ping LUO
;
Jiang ZHENG
- Publication Type:Journal Article
- Keywords:
chloroquine;
Escherichia coli;
DNA, bacterial;
interlukin-6;
Toll-like receptors;
NF-kappa B
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to explored the mechanism of inhibitory effect of chloroquine on IL-6 release induced synergistically by bacterial DNA and endotoxin. Before experiment mice were sensitized for 1 hour with D-GalN by I.P. injection. Mortality within seven days was observed after mice were challenged with CpG ODN or LPS with or without chloroquine treatment. ANA-1 cell line cells were cultivated in vitro. We investigated the influence of chloroquine on IL-6 release induced by both EC DNA and LPS. Expressions of TLR4 and TLR9 and activation of NF-?B p65 were investigated in cultured THP-1 cells pretreated with chloroquine and stimulated by EC DNA and LPS. The results showed that all mice died within 4 hours after challenged with CpG ODN and LPS. Only 4 or 5 mice pretreated with chloroquine (30mg/kg) died after challenged with CpG ODN and LPS (P
