PROTECTIVE EFFECTS OF MELATONIN ON CALF AORTIC ENDOTHELIAL CELLS IN MEDIUM WITH HIGH CONCENTRATION OF GLUCOSE
- VernacularTitle:褪黑素对高糖条件下牛主动脉内皮细胞保护作用的实验研究
- Author:
Hui ZHANG
;
Libin ZHANG
;
Zhimin LIU
- Publication Type:Journal Article
- Keywords:
melatonin;
aorta;
endothelial cells
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
To observe the effects of melatonin on postacyclin concentration, nitric oxidase activity(NOS) and inducible nitric oxidase(iNOS) mRNA expression in calf aortic endothelial cells in medium with high concentration of glucose, endothelial cells of calf thoracic arota were cultured and divided into five groups. Group A was a low-glucose control with 5.5mmol/L glucose in medium. Group B was a high-glucose control with 33mmol/L glucose in medium. Cells of group C was cultured with 33mmol/L glucose and 10 -13mol/L melatonin, those of group D was cultured with 33mmol/L glucose and 10 -9mol/L melatonin, and those of group E was cultured with 33mmol/L glucose and 10 -5mol/L melatonin. The concentration of 6-keto-PGF 1?, metabolizing product of PGI 2, in the culture medium was measured with radio-immunological assay. NOS activity was determined with colorimetry and iNOS mRNA was measured with semi-quantitative RT-PCR. 6-keto-PGF 1? concentration, NOS activity and iNOS mRNA expression in group A were significantly lower than those in group B(P0.05). 6-keto-PGF 1? concentration of group D and group E was significantly higher than that in group B(P
