STUDY OF EXPRESSION OF ECE-1 mRNA IN GASTRIC MUCOSA OF COLD-RESTRAINT-STRESS RATS
- VernacularTitle:应激状态下胃粘膜组织ECE-1 mRNA表达变化及其意义
- Author:
Yimin DUAN
;
Zhaoshen LI
;
Xianbao ZHAN
- Publication Type:Journal Article
- Keywords:
stress;
gastric mucosal lesion;
endothelin-1;
endothelin-1-converting-enzyme;
reverse transcriptase polymerase chain reaction
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
To explore the significance of endothelin-1 converting enzyme (ECE-1)mRNA expression in the development and progression of stress ulcer in cold-restraint-stress(CRS) rats, a model was established by CRS-induced ulcers in rats. ET-1 contents in plasma and gastric mucosa of rats were measured by using radioimmunoassay(RIA)method, gastric mucosa blood flow(GMBF) in rats were determined by using laser-doppler flowmetry, ulcer index(UI) in rats was estimated according to"Guth standard", and the expression levels of ECE-1mRNA in gastric mucosa of normal and CRS rats were measured by using dot blot and reverse transcription-polymerase chain reaction(RT-PCR). The results indicated that compared with control values,the ET-1 contents in both plasma and gastric mucosa of CRS rats were increased significantly after stress (P
