RELATION BETWEEN CX43 EXPRESSION AND APOPTOSIS AND PROLIFERATION IN DEVELOPMENT OF STRESS ULCER
- VernacularTitle:CX43在应激性溃疡发生发展过程中的表达与细胞凋亡、增殖的关系
- Author:
Jing LIU
;
Zhaoshen LI
;
Xinjian WAN
- Publication Type:Journal Article
- Keywords:
CX43;
ulcer;
stress;
apoptosis;
proliferation
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
To determine the effect of CX43 expression on gastric mucosa apoptosis and proliferation under stress conditions, a water immersion-restraint stress(WRS) model was performed with SD rats. Apoptotic cells were quantitated in gastric mucosa by terminal deoxynucleatidyl transferase-mediated dUTP nick and labelling (TUNEL) techniques. Cell proliferation was detected by immunohistochemical staining for proliferating cell nuclear antigen (PCNA). The expression of CX43 mRNA was detected by in situ hybridization. The results revealed that in control rats not exposed to WRS, apoptotic cells were seen only occasionally at the surface epithelium, The PCNA cells occurred in the proliferation zone of the gastric glands. The CX43 mRNA predomination occurred in the zone of the gastric glands. In contrast, the apoptotic quantities rose significantly 2h to 5h after the termination of WRS and reach a peak gradually, while PCNA proteins and the CX43 mRNA decreased significantly. At 8～12h after WRS the apoptotic cells gradually declined, while the PCNA cells increased to reach a peak. The CX43 mRNA almost returned to normal level and then increased to reach a peak. At 24h after WRS, the apoptotic cells remained significantly higher than normal level while the expression of PCNA proteins and CX43 mRNA returned to the values observed in the intact rats.These results suggested the expression of CX43 might associate with cellular differentiation, proliferation and the stabilization of glandular zone and affect the apoptosis of the surface epithelium.
