The effect of idazoxan on pancreatic ?-cells and its mechanism
- VernacularTitle:咪唑克生对胰岛细胞的损伤效应及其机制
- Author:
Hongwei GAO
- Publication Type:Journal Article
- Keywords:
idazoxan, BRIN-BD11 cells, apoptosis
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
AIM To investigate the effects of idazoxan on the viability and DNA integrity of clonal BRIN-BD11 cells. METHODS MTS reduction was used to check the viability of BRIN-BD11 cells. FACS cell cycle analysis was employed to determine the effects of idazoxan on DNA integrity of BRIN-BD11 cells. RESULTS ①Idazoxan markedly reduced the viability of BRIN-BD11 cells and resulted in DNA damage in time-and dose-dependent manner.②Genistein, 5-HT 1A antagonist NAN190 and PKG inhibitor-KT5823 had no protective effects against idazoxan-induced damage of BRIN-BD11 cells. CONCLUSION Idazoxan has cytotoxic effect and induces apoptosis of clonal pancreatic ?-cells.
