The experimental studies of the effects of As_2O_3 on the expression of telomerase hTERT-mRNA and apoptosis of HL-60 cells
- VernacularTitle:三氧化二砷对HL-60细胞凋亡及其端粒酶hTERT-mRNA表达影响的实验研究
- Author:
Yao ZHANG
;
Yan ZHAO
;
Changjie CHEN
- Publication Type:Journal Article
- Keywords:
human telomerase reverse transcriptase(hTERT);
apoptosis;
HL-60 cells;
arsenic trioxide
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effects of apoptosis and the expression of telomerase hTERT mRNA activity of HL-60 cells treated with As 2O 3 in different concentrations. METHODS HL-60 cells was cultured with As 2O 3 in different concentrations for 48 hours, then, cell apoptosis was detected with flow cytometry and the expression of telomerase hTERT mRNA was determined with RT-PCR respectively. RESULTS The percentage of the apoptosis in HL-60 cells were 2 28%?0 40%?2 55%?0 22% and 47 57%?2 79% respectively, as the cells were treated with 0 1 ?mol?L -1,1 0 ?mol?L -1,5 0 ?mol?L -1 As 2O 3 for 48 hours. The difference was significant between 5 0 ?mol?L -1 and 0 1～1 0 ?mol?L -1 groups(P
