IL-1? and TNF-? gene expression in periapical lesions of immuno-suppressed rat
- VernacularTitle:环磷酰胺对鼠根尖周炎中IL-1?、TNF-?基因表达的影响
- Author:
Lingxuan MEI
;
Zheng LIU
;
Bi ZHANG
- Publication Type:Journal Article
- Keywords:
periapical lesion;
IL-1?;
TNF-?;
gene expression;
immunosuppression
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM The purpose of this study was to investigate the effect of immunosuppression on the periapical inflammation by estimating the expression of the cytokine IL-1? and TNF-? in periapical lesion of rat. METHODS Sixteen SD rats received cycolophosphamide injection weekly before pulp exposure for three weeks, another 16 animals received no medication as control group. The expressions of IL-1? and TNF-? mRNA in periapical lesion were detected by methods of in situ hybridization,and semi-quantified analyzed compared with control group. RESULTS IL-1? and TNF-? mRNA expressions in treated group increased progressively from 1 to 3 week, and declined somewhat at 4 week. There was a statistically significant positive correlation between the area of the periapical lesion and the number of positive stained cell for IL-1? and TNF-?(IL-1?:r=0 881,P
