Establishment and application of HCV genotype liquichip detection method
10.3969/j.issn.1673-4130.2014.13.019
- VernacularTitle:丙型肝炎病毒核酸液相芯片分型检测方法的建立及应用
- Author:
Youliang ZHOU
;
Chunling HU
;
Zhaohui WANG
;
Chuanlu REN
;
Ping XU
;
Peiqin CHEN
;
Xing LIU
- Publication Type:Journal Article
- Keywords:
hepatitis C;
hepatitis viruses;
liquichip;
polymerase chain reaction
- From:
International Journal of Laboratory Medicine
2014;(13):1710-1712,1715
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a liquichip method for detecting 6 sub-genotypes of hepatitis C virus(HCV),including 1a, 1b,2a,3a,3b and 6a.Methods The coupling method of PCR amplification and nucleic acid probe was established.The PCR product and the microspheres mixture of the coupled nucleic acid probe were hybridized for establishing the liquichip detection method.The sensitivity and specificity of the established liquichip detection method were evaluated.Nucleic acid in 93 serum samples was detec-ted by this method..Results The established HCV nuclei acid liquichip genotype detection method had the higher specificity and sensitivity,which could detect and classfy 6 HCV sub-genotypes.The sensitivity for HCV 1a,3a and 6a sub-genotypes was 1× 105 copies/PCR;the sensitivity for HCV 1b,2a and 3b sub-genotypes was 1×104 copies/PCR.The detection results in 93 serum samples showed that the this genotyping method had the characteristics of high throughput,rapidness,sentsitivity and specificity. Conclusion This method can be used for the simultaneous and quick detection of 6 HCV sub-genotypes and provides a new meth-od for the genotyping detection of HCV.
