EFFECT OF LIPOPOLYSACCHARIDE ON EXPRESSION OF INTERLEUKIN-6 IN COCULTURED LUNG MICROVASCULAR ENDOTHELIAL CELLS
- VernacularTitle:脂多糖对复合培养的肺微血管内皮细胞表达IL-6的影响
- Author:
Guansong WANG
;
Guisheng QIAN
;
Guomin WU
- Publication Type:Journal Article
- Keywords:
lipopolysaccharides;
coculture;
pulmonary artery;
endothelium, vascular;
muscle, smooth, vascular;
interleukin 6
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Rat lung microvascular endothelial cells (LMVEC) were cocultured with rat pulmonary arterial smooth muscle cells (PASMC). The cultures were divided into 5 groups: normal group (N), lipopolysaccharide (LPS) 2h (L 2), LPS 8h (L 8), LPS 16h (L 16 ), and LPS 24h (L 24 ). The activity of IL 6 in LMVEC and PASMC supernatants was determined by radio immunity method, and the expression level of IL 6 mRNA in LMVEC was detected with RT PCR. The results showed that the activity of IL 6 in LMVEC and PASMC was enhanced by LPS at L 2, reaching the higher level after 8 hour stimulation. Furthermore, the activity of IL 6 was stronger in homotypic groups than that of coculture groups. The expression of IL 6 mRNA in LMVEC was increased in L2 group, and highest in L6 group, but lowered in L12 group, though it was still stronger than that of N. It suggest that IL 6 mRNA and the activity of IL 6 in LMVEC and PASMC were enhanced in both homotypic and coculture groups by LPS. And the mRNA level and activity of IL 6 were higher in homotypic groups than that of coculture groups.
