EFFECTS OF LIPOPOLYSACCHARIDE ON ?-ADRENOCEPTOR AND ?-ADRENOCEPTOR KINASES IN RAT PULMONARY MICROVASCULAR ENDOTHELIAL CELLS
- VernacularTitle:脂多糖对肺微血管内皮细胞?-受体及?-受体激酶影响的研究
- Author:
Zhenliang XIAO
;
Guisheng QIAN
;
Gengyu SUN
- Publication Type:Journal Article
- Keywords:
receptors, adrenergic, beta;
G protein coupled receptor kinase;
microvascular endothelial cell, pulmonary;
rats;
lipopolysaccharides
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to probe the effects of lipopolysaccharide(LPS) on ? adrenoceptor (? AR) and ? AR kinases in rat pulmonary microvascular endothelial cells (RPMVEC), radioactive ligand binding assay was used to measure the distribution density of ? AR before and after LPS acting in it; Western blot was used to observe the expression changes in ? AR kinases, including GRK2 and GRK3. The results showed that the maximum binding capacity (Bmax) of RPMVEC ? AR was 5 583?0 306 fmol/10 5 cells. The Bmax of ? AR in LPS group significantly decreased compared with normal control group ( P
