EXPRESSION AND FUNCTION OF THE ?-ADRENOCEPTORS IN RAT DETRUSOR MUSCLE
- VernacularTitle:肾上腺素?受体在大鼠逼尿肌中的表达及功能
- Author:
Wenfeng CAO
;
Bo SONG
;
Xiy JIN
- Publication Type:Journal Article
- Keywords:
rats;
receptors, adrenergic, beta;
detrusor
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the expression of ? adrenoceptors(? AR) and its possible function in rat detrusor. The relaxation response produced by ? AR subtypes(? 1 , ? 2 , ? 3 AR) agonists or antagonists were examined by means of the in vitro detrusor strip study, and the expression of ? AR subtypes mRNA was investigated in rat detrusor muscle by RT PCR. The selective ? 1 agonist T 0509, ? 2 agonist salbutamol, ? 3 agonist BRL37344A depressed the contractions of the detrusor muscle induced by KCl. The relaxant response to isoproterenol was antagonized by the ? 1 ,? 2 AR antagonists(100?mol/L) and the ? 3 AR antagonists SR59230(1?mol/L).RT PCR, showing that all three subtypes of ? AR subtypes mRNA were expressed in rat detrusor smooth muscles, in which ? 3 AR mRNA occupied 70%. All the ? 1 ,? 2 ,? 3 adrenoceptors participate in mediating relaxation of rat detrusor, among them ? 3 AR is the main one.
