INHIBITORY EFFECT ON ANGIOGENESIS AND GLIOMA GROWTH OF TUMOR-BEARING RATS OF VEGF ANTISENSE OLIGONUCLEOTIDE
- VernacularTitle:VEGF反义寡核苷酸抑制荷瘤大鼠胶质瘤血管生成的作用和效果
- Author:
Weifang LI
;
Guangji ZHANG
;
Chen ZHU
- Publication Type:Journal Article
- Keywords:
oligonucleotide, antisense;
glioma;
vascular endothelial growth factor;
inhibition;
rats
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
To observe the inhibitory effects of VEGF antisense oligonuoleotide on angiogenesis and glioma growth of tumor bearing rats. 1?10 6 C 6 glioma cells(20?l) were seeded with high flow microinfusion into right caudate nucleus of all rats with sterotactic technique. In the treatment groups Ⅰ and Ⅱ 1 000 ?mol/L, 2 000 ?mol/L VEGF antisense oligonucleotide was respectively given at the site of seeding twice 1 and 2 weeks after cell inoculation. The control group received only 20?l Hank′s solution twicely. Three weeks after cell inoculation, the general condition of the treated rats were better, while rats in control group were in precarious conditions. The inhibition rate of tumor growth was 91 5% in the treated group Ⅰ, 100% in the treated group Ⅱ. The expressions of VEGF mRNA, VEGF, and MVD were significantly different between treatment and control groups. The results indicated that VEGF was required for the maintenance of angiogenesis in the tumor, and inhibition of VEGF might be a therepeutic strategy in the treatment of glioma.
