CLONING OF HUMAN ANGIOPOIETIN-1 GENE AND CONSTRUCTION OF ITS EUKARYOTIC AND PROKARYOTIC EXPRESSION VECTORS
- VernacularTitle:人血管生成素-1基因的克隆及原核、真核表达载体的构建
- Author:
Jun WANG
;
Kaichun WU
;
Dexi ZHANG
- Publication Type:Journal Article
- Keywords:
angiogenesis;
angiopoietin;
tumor;
polymerase chain reaction;
gene cloning
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Angiopoietin is a novel angiogenesis related molecule. To investigate its roles in tumor angiogenesis and metastasis,angiopoietin 1 gene was obtained from human placental tissue by reverse transcription polymerase chain reaction (RT PCR). The sense, antisense eukaryotic expression vector pcDNA3.1/hangiopoietin1(+), antisense eakaryotic expression vector pcDNA3.1/hangiopoietin1(-), and prokaryotic expression vector pRSETC hangiopoietin1 were successfully constructed by directional cloning the target gene into pcDNA3.1 V5 His C and pRSET C vectors, respectively. This lays good foundation for further study on the roles of angiopoietin 1 in angiogenesis in tumors of the digestive tract.
