EFFECT OF INTERACTION BETWEEN p53 AND HBV ON CELL APOPTOSIS AND p21 PROMOTER IN HEPATOMA CELL LINE 7721
- VernacularTitle:p53与HBV相互作用对7721细胞凋亡及p21启动子的影响
- Author:
Jianhui QU
;
Minghua ZHU
;
Jin LIN
- Publication Type:Journal Article
- Keywords:
genes, p53;
hepatitis B virus;
genes, p21;
liver neoplasms;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
To observe whether there is an interaction between hepatitis virus B (HBV) and tumor suppressor p53, plasmid PCMVp53 was transfected or cotransfected with pCMVHBV a (wild type HBV) or PCMVHBV b (mutation type HBV) into the hepatoma cell line 7721 by phosphate calcium precipitation. Apoptosis cells were labeled by annexin Ⅴ FITC and detected by flow cytometry. Another experiment was performed by cotransfecting the cells with reporter plasmid p21 luc in each group mentioned above, then the luciferase activity was measured. The results showed that the cells transfected by pCMVp53 alone exhibited high luciferase activity and high apoptosis rate. Meanwhile, the luciferase activity and apoptosis rate were further higher in cells cotransfected by pCMVp53 and PCMVHBV a , but remained unchanged in cells cotransfected by PCMVp53 and PCMVHBV b. The results indicated that P53 could induce of 7721 cell apoptosis by activating p21 transcription, and such effect could be enhanced by HBV.
