SCREENING AND CLONING OF GENE OF HEPATOCYTE PROTEIN INTERACTING WITH HCV NS3 PROTEIN
- VernacularTitle:酵母双杂交技术筛选克隆丙型肝炎病毒NS3结合蛋白基因
- Author:
Ke LI
;
Lin WANG
;
Ju CHENG
- Publication Type:Journal Article
- Keywords:
Hepatitis C virus;
NS3 protein;
yeast two hybrid;
protein protein interaction
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
To clone the genes of hepatocyte protein interacting with hepatitis C virus NS3 protein, "bait" plasmids of hepatitis C virus NS3 were constructed. After verifying that hepatitis C virus NS3 protein could be steadily expressed in AH109 yeast strain, yeast two hybrid assay was berformed by mating AH109 with Y187 that pre transformed with liver cDNA library plasmids pACT2, and the diploidy yeast cells were plated on quadruple dropout (QDO) medium and assayed for X ? gal activity. Nineteen yeast colonies that could grow on QDO and had ? gal activity were obtained, then the library plasmids were extracted and sequenced. The gene sequences from the 19 positive colonies were aligned with the genes deposited in GenBank. It was found hepatitis C virus NS3 protein could interact with some proteins which have different functions.
