EPITOPE MAPPING OF HEPATITIS C VIRUS CORE PROTEIN FROM A PEPTIDE PHAGE LIBRARY BY USING IMMOBILIZED SPECIFIC MONOCLONAL ANTIBODY
- VernacularTitle:应用噬菌体表面展示技术筛选丙型肝炎病毒核心蛋白抗原模拟表位
- Author:
Yanwei ZHONG
;
Jun CHENG
;
Gan WANG
- Publication Type:Journal Article
- Keywords:
Hepatitis C virus;
core protein;
mimotopes;
phage peptide library
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
To screen HCV core mimotopes,HCV core monoclonal antibody was used as immobilized molecule,and a 12 mer phage peptide library was biopanned and positive clones were selected by enzyme linked immunoadsorbent assay(ELISA), competitive inhibition assay, and DNA sequencing. 10 positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results,one epitope was confirmed as a mimotope of HCV core protein. The study might provide a new approach for HCV therapy and vaccine development.
