THE STUDY OF TRANSACTIVATING EFFECT OF HCV NS3 PROTEIN ON SV40 EARLY PROMOTER
- VernacularTitle:丙型肝炎病毒非结构蛋白NS3反式激活SV40病毒早期启动子的研究
- Author:
Yan LIU
;
Jun CHENG
;
Jinson MU
- Publication Type:Journal Article
- Keywords:
hepatitis C virus;
NS3 gene;
eucaryotic expression;
transactivating effect;
SV40 early promoter
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Hepatitis C virus (HCV) non structure 3 (NS3) gene was amplified from plasmid pBRTM3011 by employing polymerase chain reaction (PCR), and the amplified product was cloned into pcDNA3 1(-) vector. Then the recombinant plasmid pcDNA3 1(-) NS3 was transfected into HepG2 cells and was cotransfected into HepG2 cells with reporter plasmid pCAT3 promoter, respectively. HCV NS3 protein expressed in HepG2 cells was detected by reverse transcription PCR (RT PCR) and Western blotting method. The activity of CAT was detected by a ELISA kit, which reflected the transactivating function of HCV NS3 protein. The results showed that HepG2 cells transfected with pcDNA3 1(-) NS3 could express HCV NS3 protein. The expression of CAT in HepG2 cells transfected with the pcDNA3 1(-) NS3 was 4 6 fold higher than that of control plasmid. It was suggested that the recombinant plasmid pcDNA3 1(-) NS3 could be expressed in mammalian cell line, and had transactivating effect on SV40 early promoter
