SCREENING AND CHARACTERIZATION OF HUMAN PHAGE ANTIBODY TO HEPATITIS C VIRUS E2 ANTIGEN
- VernacularTitle:丙型肝炎病毒包膜蛋白E2人源单链可变区抗体的筛选与鉴定
- Author:
Yanwei ZHONG
;
Jun CHENG
;
Shuangshuan SHI
- Publication Type:Journal Article
- Keywords:
phage display technique;
HCV;
envelope protein E2;
biopanning;
scFv phage antibody
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
To screen and characterize human phage antibody to hepatitis C virus E2 antigen. The recombinant phages were panned by HCV E2 antigen which was coated in a microwell plate. After three rounds of biopanning, 56 clones specific to HCV E2 antigen were obtained. The specificity of scFv was determined by ELISA method and cross reaction with BSA and competitive inhibition assay. HCV E2 phage antibody had a specific combination character with recombinant hepatitis C virus E2 antigen. The DNA sequence data showed that the scFv coding gene was 771bp in size
