SCREENING OF GENES TRANSREGULATED BY CORE PROTEIN OF HEPATITIS C VIRUS WITH cDNA MICROARRAY
- VernacularTitle:应用基因表达谱芯片筛选丙型肝炎病毒核心蛋白反式调节基因
- Author:
Yan LIU
;
Jun CHENG
;
Jianju WANG
- Publication Type:Journal Article
- Keywords:
hepatitis C virus;
core protein;
transregulation;
cDNA microarray
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to screen genes transregulated by core protein of hepatitis C virus, cDNA microarray technology was employed to detect the gene expression change between HepG2 cells transfected with pcDNA3 1(-) core and the empty vector, respectively. Among 1152 genes, there were 95 genes with different expressions, of which 45 genes were upregulated and 50 genes were downregulated in HepG2 cells transfected with core protein expression plasmid. These genes transregulated by HCV core protein included human genes encoding proteins involved in cell proliferation, differentiation, apoptosis, signal transduction and immune regulation. Therefore, the results provided some new clues for further clarifying the molecular biology mechanism of pathogenesis and tumorigenesis of HCV core protein
