THE EFFECTS OF MONOAMINE ON CULTURED NEURONS
- VernacularTitle:单胺类递质对神经细胞的影响
- Author:
Wenjun QU
;
Ruxiang XU
- Publication Type:Journal Article
- Keywords:
biogenic amine neurotransmitters;
apoptosis;
neurons
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
To study the toxicity of monoamine in high concentrations to neurons. Neurons were treated in vitro with different doses of dopamine, norepinephrine and serotonin for various lengths of time. The toxicity of these reagents on neurons was observed. Apoptosis of neurons was analyzed by agar electrophoresis, in situ DNA end labeling technique and flow cytometry. The results showed that a high concentration of monoamine could induce apoptosis of the culturing neurons. At 24h after treatment with dopamine or norepinephrine (80, 160 and 320?mol/L) as well as with 320?mol/L serotonin, apoptotic rates of neurons were significantly higher than that of the controls, respectively ( P
