THE EFFECT OF HEPARIN AND PROTAMINE ON THE EXPRESSION OF PLATELET MEMBRANE GLYCOPROTEIN
- VernacularTitle:肝素、鱼精蛋白对血小板膜糖蛋白影响的实验研究
- Author:
Bo SUI
;
Maoyuan LI
- Publication Type:Journal Article
- Keywords:
platelets;
cardiopulmonary bypass;
heparin;
protamines
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study is to invistigate the effects of heparinization and the reversal of heparin activity with protamine on platelet function during extracorporeal cardiopulmonary bypass. The venous blood of 10 healthy volunteers was collected in tubes containing 3 2% trisodium citrate or 25??/ml heparin. The expression of P selectin (a secretion marker of platelets) and activated fibrinogen receptor GP Ⅱb Ⅲa (an aggregation marker of platelets) on normal platelets in response to the treatment with heparin and protamine in vitro were examined using whole blood flow cytometry. The results showed that heparin (5～50?g/ml) could increase adenosine diphosphate (ADP) induced expression of P selectin and GPⅡb Ⅲa in a dose dependent manner ( P
