The effect of topotecan on lung cancer cell cycle and the mechanism of topotecan-induced apoptosis
- VernacularTitle:拓扑替康对肺癌细胞生长周期的影响及诱导凋亡的作用机制
- Author:
Tao JIANG
;
Kun LIU
- Publication Type:Journal Article
- Keywords:
topotecan;
lung cancer;
cell cycle;
apoptosis;
caspase 3;
bcl 2
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the effect of Topotecan(TPT) on cell cycle and apoptosis of hunman lung cancer SPC A 1 cells and the role of Caspase 3 and bcl 2 involved in TPT induced apoptosis. METHODS Cells were incubated with TPT(5,10,15 and 20 mg?L -1 ) for 24 h. Apoptosis was detected by TUNEL staining, electronic microscope and DNA agarose gel electropheresis. Flow cytometer was used to analyse cell cycle changes and to detect apoptotic rates and bcl 2 expression of cells treated with TPT or TPT and 50 ?mol?L -1 Ac DEVD CHO(specific Caspase 3 inhibitor). RESULTS Apoptosis of cells treated with TPT(5 mg?L -1 ) was not detected, but apoptosis rates increased with the increase of TPT concentration and there was significant difference between each concentration group( P
