Effect of antisense phosphorothioate oligonucleotide on activity of telomerase for human bladder tumor cell
- VernacularTitle:反义硫代寡核苷酸对人膀胱癌细胞端粒酶活性的影响
- Author:
Zhiyu LIU
;
Yongji LIU
;
Yuyan LI
- Publication Type:Journal Article
- Keywords:
AsONS;
Human bladder tumor;
Telomerase
- From:
Journal of Clinical Surgery
2002;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the Telomerase inhibitor-antisense phosphorothiate oligonucleotide (asONS) effect on telomerase activity of human bladder tumor cell line BIU-87.Methods BIU-87 cells and cell extracts are cultured with asONS,non-asONS and control fluid at different concentrations and times respectively.Telomerase activity was assayed by TRAP PCR-ELASA.Results AsONS can inhibit the activity of BIU-87 cells ,whereas non-asONS and control group not, and have significant difference(P
