Delayed protective effect of liver ischemic preconditioning on liver ischemia-reperfusion injury and initial inquiry into the protective mechanism
- VernacularTitle:缺血预处理对大鼠肝脏缺血再灌注损伤的延迟性保护作用及其机制的初步探讨
- Author:
Xiaoling ZHENG
;
Jiling YI
;
Limo CHEN
- Publication Type:Journal Article
- Keywords:
Ischemia preconditioning;
Liver;
Ischemic reperfusion injury;
Heat shock protein;
Tumor necrosis factor
- From:
Journal of Clinical Surgery
2002;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study evaluated whether liver ischemic preconditioning has delayed protective effect on liver ischemia-reperfusion injury and probe into the protective mechanism.Methods 36 wistar rats were randomly divided into two groups, group A:ischemic preconditioning,Group B:Control. The group A rats were subjected to liver ischemic preconditioning,While group B rats were subjected only laparotomy.After 48 hours,Liver tissue samples were abstained to detect the expression of HSP70.Occlusion the inflow vessels to the left and median lobes 45-minute then reperfuse 90-minute.Liver tissue samples were abstained to detect the expression of TNF-?.Serum ALT was detected before ischemia and after 90-minute reperfusion.Results The serum ALT level in group A is much lower than that in group B (P
