STUDY OF GENES DIFFERENTIALLY EXPRESSED IN CD34~+ CELLS FROM G-CSF-MOBILIZED PERIPHERAL BLOOD AND BONE MARROW USING THE TECHNIQUE OF SUPPRESSION SUBTRACTIVE HYBRIDIZATION
- VernacularTitle:骨髓和动员外周血中CD34~+细胞基因表达差异的研究
- Author:
Jie CHEN
;
Shuyun ZHOU
;
Ru FENG
- Publication Type:Journal Article
- Keywords:
hematopoietic stem cell transplantation;
suppression subtractive hybridization;
antigens, CD34
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Our aim in this study was to determine differentially expressed genes of CD34 + cell from bone marrow and G CSF mobilized peripheral blood. CD34 + cells isolated from bone marrow and G CSF mobilized peripheral blood of the same donor were identified for differentially expressed genes in CD34 + cells using the technique of suppression subtractive hybridization. Eleven genes were expressed with higher levels in CD34 + cells from mobilized peripheral blood, as compared with data of GenBank. These genes included nuclear proteins, transcriptional regulatory molecules, zinc finger proteins and interferon induced molecules. These results demonstrate that there are some differences between the two groups of CD34 + cells. Further study would give us more extensive understanding about mobilization and homing of hematopoietic stem cells.
