Biological characters of hepatitis B virus transgenic mice strain C57-TgN(HBV adr2. 0)SMMU
- VernacularTitle:乙型肝炎转基因小鼠品系C57-TgN(HBV adr2.0)SMMU的生物学特征
- Author:
Xiaoyuan ZI
;
Yucheng YAO
;
Jun XIONG
;
Yanhua JIN
;
Xuting YE
;
Jianxiu LI
;
Hong LIU
;
Haiying ZHU
;
Xinmin WANG
;
Wenjun NI
;
Hongyu YU
;
Wenming CONG
;
Yiping HU
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
transgenic mouse;
immunohistochemistry;
pathology
- From:
Academic Journal of Second Military Medical University
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the biological characters of C57-TgN(HBV adr2.0)SMMU transgenic mice. Methods: Integration,expression,replication and histology change of hepatitis B virus gene in F6 transgenic mice were estimated by ge-nomic DNA PCR,Western blotting,ELISA,immunohistochemistry,serum DNA PCR,transmission electron microscopy and H-E staining. Results: Hepatitis B virus gene was integrated into F6 C57-TgN(HBV adr2. 0)SMMU transgenic mice and expressed HBsAg,HBcAg and X protein in liver tissue. HBsAg and HBeAg were expressed in serum of 19. 54% and 3. 39% F6 transgenic mice. Hepatitis B virus were replicated in serum and liver tissue of transgenic mice. Long-term integration,expression and replication of hepatitis B virus gene induced pathological lesion of transgenic mice liver and lung. Conclusion: C57-TgNCHBV adr2. 0)SMMU transgenic mice line has the biological characters including integration of hepatitis B virus gene into genomic DNA,expression and replication of hepatitis B virus gene in serum and liver, and histological change in liver and lung. It is a valuable animal system to study pathogenesis, treatment and prevention of hepatitis B virus.
