Clone of porcine IL-4 cDNA and its application in DNA vaccine of Cysticercus cellulosae
- VernacularTitle:猪白细胞介素4的基因克隆及其在囊尾蚴DNA疫苗中的应用
- Author:
Dan WU
;
Yingjun GUO
;
Qingmin WANG
;
Shuhan SUN
- Publication Type:Journal Article
- Keywords:
porcine IL-4;
gene clone;
Cysticerus cellulosae;
DNA vaccine;
adjuvanticity
- From:
Academic Journal of Second Military Medical University
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To clone porcine IL-4 cDNA and observe its adjuvanticity of vaccine against T. solium cysticerco-sis. Methods:The cDNA of porcine IL-4 was amplified by RT-PCR, which had 5' AUG initiatory codon with optimized trans-lational initiation. After sequencing,the cDNA was intergrated into expression vector pcDNA and transient expression was performed. Then newborn pigs were immunized with IL-4 expression vector and protective antigen DNA vaccine against T. solium cysticercosis. Results:The obtained sequence of porcine IL-4 cDNA was the same as reported. IL-4 and protective antigen could effectively promote the humoral response. Conclusion:An efficient expression plasmid containing porcine IL-4 cDNA is constructed. Its adjuvanticity of DNA vaccine against T. solium cysticercosis is preliminarily conformed.
