Highly effective transformation of Cryptococcus neoformans
- VernacularTitle:新生隐球菌高效转化方法的研究
- Author:
Weihua PAN
;
Wanqin LIAO
;
Julin GU
;
Keke HUO
- Publication Type:Journal Article
- Keywords:
Cryptococcus neoformans;
transformation;
chemical transformation;
electrotransformation
- From:
Academic Journal of Second Military Medical University
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a highly effective transformation method of Cryptococcus neoformans. Methods: Special reagents was used to make C. neoformans take in external DNA under given condition. This chemical transformation result was compared with that of electrotransformation. The feasibility of this chemical transformation was tested by plasmid stability test. Results: The efficiency of this chemical transformation was more than 103 transformants/?g plasmid DNA, far more than that of the traditional electrotransformation. Conclusion: An appropriate transformation method is established for C. neoformans transformation, which has high transformation efficiency.
