STUDY ON CULTURE AND DIFFERENTIATION OF BMSC FROM MACHIN MACACA LRUS
- VernacularTitle:食蟹猴骨髓基质干细胞体外诱导分化的实验研究
- Author:
Gang LI
;
Yiquan KE
;
Xiaoda JIANG
- Publication Type:Journal Article
- Keywords:
bone marrow stroma cells;
stem cell;
cell cultures;
Macaca Lrus
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
To observe the growth, expansion and differentiation of the cultured bone marrow stroma cell (BMSC) from Macaca Lrus, BMSC isolated from adult Macaca Lrus were cultured with the culture medium confected by ourselves and were induced with some cytokines such as LIF and bFGF. The results showed that the BMSC could proliferate and generate Nestin positive clones when they were cultured in vitro. After subculture, these cells could grow rapidly and differentiated into neuron like cells and astrocyte like cells further, which expressed GFAP or NSE antigen respectively. Therefore, these BMSC possess renewal and differentiation abilities. On the other hand, the culture method we used in this experiment is suitable for culture of BMSC in vitro. The BMSC might be used as the seed cells of the neural stem cells.
