ESTABLISHMENT OF A GASTRIC CANCER CELL MODEL WITH HIGH EXPRESSION OF ZINC RIBBON PROTEIN GENE ZNRD1 BY MICROINJECTION
- VernacularTitle:锌带蛋白基因ZNRD1高表达胃癌细胞模型的建立
- Author:
Yumei ZHANG
;
Quanjian YAN
;
Yongqua SHI
- Publication Type:Journal Article
- Keywords:
zinc ribbon protein;
gastric cancer;
cell model;
ZNRD1 gene
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
To construct a eukaryotic vector and establish a gastric cancer cell model highly expressing zinc ribbon protein gene ZNRD1, ZNRD1 cDNA was inserted into multiple cloning sites of the pcDNA3 1 + with molecular cloning technique. The recombinant vector was identified by endonuclease digestion and transfected into SGC7901 cells by nucleus microinjection. Northern blot was used to detect the expression of ZNRD1 in cells. The results showed that ZNRD1 was successfully cloned into pcDNA3 1 + and microinjected into SGC7901. Therefore, a gastric cancer cell model highly expressing ZNRD1 has been established and can be used for further functional research of this gene.
