ASSOCIATION BETWEEN DIFFERENT REFLUX IN ESOPHAGUS AND mRNA EXPRESSIONS OF p53,p16 AND p21 DURING ESOPHAGEAL TUMORIGENESIS OF RATS
- VernacularTitle:食管反流促进肿瘤发生与抑癌基因mRNA表达的相关性
- Author:
Wen WANG
;
Zhaoshen LI
;
Guoming XU
- Publication Type:Journal Article
- Keywords:
gastroesophageal reflux;
esophageal neoplasm;
rats;
genes, p53;
genes, p16;
genes, p21;
RNA, messenger
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the mRNA expressions of p53,p16 and p21 in esophagus under duodenoesophageal reflux and gastroesophageal reflux and to discuss the mechanism of reflux leading to esophageal tumorigenesis. Three kinds of model groups [gastroesophageal reflux(G), duodenoesophageal reflux(D) and duodenogastroesophageal reflux(DG)] and a control group(C) were made by operations. All rats were given carcinogen and the mRNA expressions of p53, p16 and p21 were measured by in situ hybridization. The expression of p53 mRNA in all reflux groups was significantly more than that in group C, and in groups D and DG was significantly more than that in group G. The expressions of p16 and p21 gene decreased in all reflux groups and were significantly lower than those in group C, but there was no difference between these reflux groups. It is concluded that reflux of both gastric juice and duodenal contents can promote tumourigenesis in esophagus by changing the expressions of neoplasm inhibiting genes.The effect of duodenal contents is stronger than that of gastric juice.
