REGULATION OF PRECONDITIONING ON EXPRESSION OF PROTO-ONCOGENES c-fos, c-jun IN ISCHEMIA-REPERFUSION CARDIOMYOCYTES
- VernacularTitle:预处理对缺血再灌注心肌细胞即刻早期基因c-fos、c-jun表达的调控
- Author:
Lihui WU
;
Zhifei XU
;
Xuewe ZHAO
- Publication Type:Journal Article
- Keywords:
cardiomyocytes;
ischemic preconditioning;
reperfusion injury;
protein kinase C;
c fos;
c jun
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
To probe into the mechanism of c fos, c jun expression regulated by preconditioning, we observed protein kinase C (PKC) changes of c fos, c jun gene transcription in cultured cardiomyocytes affected by transient ischemic and medicine preconditioning mimicking ischemia reperfusion injury. Cultured rat cardiac myocytes were exposed to ischemia reperfusion mimicking solution after brief ischemic episodes or PMA, norepinephrine, adenosine preconditioning. Using Northern hybridization assay, we detected the gene transcription of c fos, c jun in cardiomyocytes. In mimicking ischemia the content of c fos, c jun mRNA increased significantly. Drug preconditioning induced cardiomyocyte c fos ,c jun expression in varing degrees. H 7 blocked this preconditioning effect. These results suggest that through activating PKC preconditioning induced c fos, c jun expression and PKC might play a central role in ischemic preconditioning.
