STUDY ON DIAGNOSTIC VALUE OF DETECTING K-ras GENE MUTATION IN STOOL FOR LARGE INTESTINAL CANCER IN THE ELDERLY
- VernacularTitle:粪便中检测K-ras基因突变对老年大肠癌诊断价值的研究
- Author:
Ziqi ZHANG
;
Jun WAN
;
Weid YOU
- Publication Type:Journal Article
- Keywords:
K ras;
feces;
oncogenes;
colorectal neoplasms;
aged
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
To study the significance of detection of K ras mutation in stool samples in the diagnosis of large intestinal cancer, stool and local tissue samples were collected from consecutively diagnosed patients, among them 23 cases suffered from large intestinal cancer, 20 cases from colorectal adenomatoid polyps and 20 healthy subjects. DNA was extracted from both the stool and the tissue, K ras gene mutations at the first and second base of codon 12 were studied by allele specific mismatch method in both of them. The results showed that K ras mutation rate in large intestinal cancer was 56 52%(13/23), which was much higher than 5%(1/20)in healthy subjects( P 0 05). There was a 92 31%(12/13)incidence of K ras mutation at the second base of codon 12 in large intestinal cancer. The results of detecting the K ras mutation were highly identical in both the stool and the tissue ( P
