Study on the relationship of the disease activity of hepatitis B virus infection and the complexity of S region quasispecies of HBV
- VernacularTitle:乙型肝炎病毒S区准种与疾病活动性的关系
- Author:
Yingxia LIU
;
Guoling HU
;
Deming TAN
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Hepatitis B, chronic;
S gene;
Quasispecies
- From:
Chinese Journal of Infectious Diseases
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the complexity of S region qusispecies in various disease stages of chronic hepatitis B virus(HBV) infection and its relation to disease activity. Methods Serum samples were obtained from 112 patients with chronic hepatitis B virus infection;22 with chronic carries(ASC),30 with chronic mild or moderate hepatitis(CH),60 with fulminant hepatitis failure(FHF). HBV qusispecies populations were separated by the single strand conformation polymorphism (SSCP) method targeted the S region and DNA sequencing analysis. Results The number of SSCP bands detected in the patients with ASC、CH and FHF was 1.45?0.13,3.70?0.22 and 5.93?0.24, respectively. There was a statistically significant difference in the number of quasispecies among various disease stages ( P
