IN VITRO STUDY OF THE INHIBITORY EFFECT OF HCPT ON RAT LYMPHOCYTE PROLIFERATION STIMULATED BY ALLOANTIGEN
- VernacularTitle:10-羟基喜树碱对异抗原刺激下大鼠淋巴细胞增殖的抑制作用
- Author:
Ming CAI
;
Bingyi SHI
;
Chunbai MO
- Publication Type:Journal Article
- Keywords:
organ transplantation;
immunosuppressive agents;
cell differentiation;
allograft rejection;
transplantation immunology;
hydro- camptothecin
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
To study the mechanism of inhibition and the effective concentration of HCPT in treatment of acute allgraft rejection, we made an in vitro model using mixed lymphocyte culture (MLC) with the stimulator lymphocytes from SD rats and the responder ones from Wistar rats. We observed the results of the inhibitory effect of HCPT on the reaction of the lymphocytic proliferation as well as the dose-effect relationship of HCPT. The results showed that HCPT at concentrations of 100?g/ml, 10?g/ml and 2?g/ml inhibited the proliferative reaction significantly, the inhibition index were 0. 734 ? 0. 085, 0. 537?0. 361 and 0. 503 ? 0. 225, respectively. The efficacy of 100?g/ml HCPT was significantly higher that of than that of both 10?g/ml (P
