EFFECTS OF MRF4 TRANSFECTION ON DIFFERENTIATION AND EXPRESSION OF MYOGENIC REGULATORY FACTORS IN CULTERED HUMAN RHABDOMYOSARCOMA RD CELLS
- VernacularTitle:NRF4转染对横纹肌肉瘤RD细胞分化及生肌调节因子表达的影响
- Author:
Guanghui LI
;
Donglin WANG
;
Zhengtang CHEN
- Publication Type:Journal Article
- Keywords:
MRF4;
rhabdomyosarcoma;
myogenic regulatory factors;
transfection
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
To study the effects of MRF4 transfection on differentiation and expression of myogenic regulatory factors of human rhabdomyosarcoma RD cells, the plasmid-MRF4 cDNA was transfected into cultured rhabdomyosarcoma RD cells with lipofectin method. The myogenic regulatory factors MRF4 and MyoD mRNA were measured with in situ hybridization and the expressions of myosin heavy chain(MHC) and a-actin in the cells were assayed with immunocytochemical method. The cell growth and morphology were observed at the same time. It was found that the morphology of differentiation increased and the growth was suppressed in RD cells after transfection. The expression of MHC and a-actin were significantly increased in RD cells after transfection, while the expressions of MRF4 and MyoD mRNA were up-regulated. It is suggested that transfection of MRF4 can induce differentiation of RD cells and up-regulate the expression of MyoD.
