STUDY ON APOPTOSIS INDUCED BY ELECTROMAGNETIC PULSE IN NEURONS OF CEREBELLAR GRANULAR LAYER
- VernacularTitle:电磁脉冲诱导小脑颗粒细胞凋亡的研究
- Author:
Meilan ZHAO
;
Dewen WANG
;
Xiaozhe CAO
- Publication Type:Journal Article
- Keywords:
radiation;
cerebellar granule neurons;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
By observing apoptosis and morphological changes in primary culture of neurons from cerebellar granular layer, the neurons were irradiated with EMP (electromagnetic pulses), the possible injury mechanism was explored. The changes in death and apoptosis of the neurons were detected by MTT and flow cytometry, respectively. The slides stained with HE and TUNNEL were examined with light and fluorescence microscopy. The results showed that not only death of the neurons occurred immediately, but also apoptosis appeared after irradiation with EMP. Our conclusion is EMP can promote necrosis and apoptosis of neurons from cerebellar granular layer at early stage, which may result from DNA injury induced by EMP.
